FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8828279
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT677030.7668878611561778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324650.3677387178180481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279750.3168794280289511No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101770.11527728110994227No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT98650.11174318346758183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119750.078.1273961
GTATCAA342750.064.177211
ATCAACG401950.054.4798243
TCAACGC405450.054.112274
CAACGCA409100.053.483725
AACGCAG423200.051.7501036
TATCAAC446200.049.237052
TCTGTCG24500.043.7116788
ACGCAGA511350.042.9919557
CGCAGAG519450.042.344478
GTCTCGC24550.039.999891
GCAGAGT556700.039.5111129
AGAGTAC506300.032.0455910-11
GAATACG38450.030.173759
CAGAGTA519600.030.02875710-11
TCTCGCT34000.029.9279612
CTCGCTC33700.029.6646563
CTGTCGC41300.028.379669
GAGTACT411000.026.84646612-13
TCGCTCT36150.026.8311464
Produced by FastQC (version 0.11.5)