FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8828279
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146400.16583073552614275No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT94550.1070990166939672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58950.051.3468441
GTATCAA151100.050.1006971
TCAACGC149400.049.323874
CAACGCA150500.049.0028845
ATCAACG153850.047.935873
AACGCAG161050.045.9171036
TCTGTCG23150.041.5969628
GTCTCGC23350.037.4377941
TATCAAC209300.035.6045882
ACGCAGA219350.034.06577
GAATACG39800.033.4539769
CGCAGAG224300.033.2857178
GTGCTAG50800.030.5531941
GCAGAGT248450.030.0013879
AGAGTAC193300.028.4440510-11
CTCGCTC33200.026.8713573
CTGTCGC42700.026.588169
TCTCGCT33250.026.4724522
AGTACGG201500.026.06231912-13
CAGAGTA208650.024.8131210-11
Produced by FastQC (version 0.11.5)