FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8789204
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT665840.7575657590835302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327090.37214974188788885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268520.3055111702948299No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95840.10904286668053215No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT93470.10634637675948813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118800.076.140421
GTATCAA341350.064.3525541
ATCAACG405000.053.8730853
TCAACGC409900.053.2435954
CAACGCA416750.052.347025
AACGCAG431250.050.6679886
TATCAAC455850.048.163762
TCTGTCG22450.043.722958
GTCTCGC22700.042.8161741
ACGCAGA522000.041.95057
CGCAGAG529600.041.3372578
GCAGAGT564250.038.8409589
AGAGTAC516450.031.671410-11
TCTCGCT31100.031.1848142
GAATACG35800.031.0742349
CTCGCTC32050.030.2604643
CAGAGTA531300.029.07863210-11
GTGGTAT48400.028.2121521
GTGCTAG47250.027.1320571
CTGTCGC40150.026.5221849
Produced by FastQC (version 0.11.5)