FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8789204
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA172470.19622937412762292No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT89460.10178396132346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147500.050.895511
GGTATCA61850.050.5652161
TCAACGC150200.048.6397484
CAACGCA150500.048.424495
ATCAACG152200.048.233183
AACGCAG161300.045.439746
TCTGTCG19350.042.3263938
GTCTCGC20950.036.5415271
TATCAAC217350.034.1849982
ACGCAGA217150.034.108337
CGCAGAG223500.033.1929328
GCAGAGT244950.030.3111829
GAATACG42600.028.8392359
AGAGTAC193300.027.63414410-11
GTGCTAG50750.027.3628181
TCTCGCT30450.025.9212072
AGTACGG203900.025.22255912-13
GAGTACG201150.024.84447912-13
CAGAGTA209600.024.80551110-11
CTCGCTC31300.024.6484643
Produced by FastQC (version 0.11.5)