FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9474368
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT112320.118551443220276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127950.056.6169471
TCAACGC144500.047.9537734
CAACGCA145650.047.615735
GGTATCA60250.047.383121
ATCAACG146900.047.2108153
AACGCAG155150.044.672766
ACGCAGA166750.041.421197
CGCAGAG169450.040.833938
TATCAAC199100.035.6994362
GCAGAGT199450.034.7213869
TCTGTCG18400.029.3990528
AGAGTAC193200.027.15196610-11
GTCTCGC17900.026.9135781
GAGCGTT20700.025.2707149
GAGTACG201550.025.2008111
CTGTCGC22350.024.7349269
AGTACGG207600.024.46855412-13
TACGGGT34550.024.273694
CAGAGTA212250.023.3007210-11
ACGGGAC18450.021.5994743
Produced by FastQC (version 0.11.5)