FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9470365
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT119890.12659490948870503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125050.057.0130161
TCAACGC144500.047.575284
ATCAACG145500.047.3701673
CAACGCA145600.047.3383875
GGTATCA61700.045.487851
AACGCAG154900.044.720456
ACGCAGA168900.041.0837947
CGCAGAG168650.040.9632158
TATCAAC197050.035.7627562
GCAGAGT200100.034.4928869
GAGCGTT20600.029.724499
TCTGTCG17500.028.1977378
AGAGTAC194000.027.0115110-11
GTCTCGC18300.026.3191871
GAGTACG203200.024.961111
TACGGGT36650.024.5015954
AGTACGG207900.024.3996522
CAGAGTA211950.022.91484610-11
ACGGTAC9350.022.2608073
CTGTCGC21700.022.1906329
Produced by FastQC (version 0.11.5)