FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9460592
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT587440.6209336582742391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285890.30219039146810267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284530.30075284929315205No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT110700.11701170497575628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116300.073.895071
GTATCAA301100.067.006511
ATCAACG401900.052.2211723
TCAACGC405250.051.77484
CAACGCA409650.051.1143075
AACGCAG423100.049.5115366
TATCAAC444650.047.427972
CGCAGAG473650.044.2525678
ACGCAGA475400.044.0521247
GCAGAGT515400.040.6796469
AGAGTAC513200.031.7360710-11
TCTGTCG16000.029.74778
GAGCGTT21900.029.612029
CAGAGTA528100.028.52549410-11
GTCTCGC17100.027.1472931
CTGTCGC18150.026.2240059
GAGTACT399400.025.85302712-13
AGTACGG209400.024.90405712-13
AGTACTT446150.024.8176312-13
TACGGGT38100.023.8960194
Produced by FastQC (version 0.11.5)