FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9460592
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT119460.12627116780852615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127850.054.749151
TCAACGC142900.047.782494
ATCAACG143300.047.565853
CAACGCA145450.046.82215
GGTATCA61500.046.7061041
AACGCAG154400.044.162836
ACGCAGA168800.040.5018967
CGCAGAG169750.040.132388
TATCAAC199450.034.68152
GCAGAGT201150.033.9842159
TCTGTCG16850.033.868158
GTCTCGC17450.028.9684431
AGAGTAC193950.027.33798210-11
CTGTCGC22550.026.6240589
GAGTACG204250.025.564311
TACGGGT35650.025.5264844
AGTACGG209650.024.7952652
GAGCGTT21650.024.1615169
CAGAGTA214150.022.64975410-11
GTACGGG270200.020.3370651
Produced by FastQC (version 0.11.5)