FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9423310
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT595730.6321876283386623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291690.3095409150287956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267630.2840084853411381No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT111360.11817503616033008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116150.074.547711
GTATCAA305650.068.8516161
ATCAACG397250.053.218253
TCAACGC401800.052.6600384
CAACGCA406600.051.9707345
AACGCAG419200.050.464076
TATCAAC441500.047.9652372
CGCAGAG469100.045.0325978
ACGCAGA473400.044.6361247
GCAGAGT514950.041.1038639
AGAGTAC510250.031.88134810-11
GAGCGTT18650.031.578989
CAGAGTA529600.028.31264710-11
TCTGTCG17450.028.2959588
GTCTCGC17450.027.3389241
CTGTCGC19850.026.0735689
GAGTACT412900.025.67465412-13
AGTACTT455300.024.97574212-13
AGTACGG201400.024.64952912-13
GAGTACG194500.024.5583861
Produced by FastQC (version 0.11.5)