FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21558786
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1721060.7983102573586472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT947140.4393290048892363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT568450.2636744017033241No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411550.19089664881872292No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT220790.10241300229057425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA337900.076.1014941
GTATCAA802100.068.198141
ATCAACG988050.055.325013
GCGTAAG24100.054.8065531
TCAACGC1007100.054.491174
CAACGCA1014300.054.0222475
AACGCAG1046300.052.4543576
GCGTAAC10800.050.6828081
TATCAAC1114950.048.9055942
ACGCAGA1171950.046.800037
CGCAGAG1185750.046.2231148
TCTGTCG73400.043.4430168
GTCTCGC68600.043.1051141
GCAGAGT1317700.041.6441469
CTGTCGC88000.036.4382369
CTCGCTC79150.036.1554373
GCGTACT23600.034.538631
TCTCGCT85900.034.0071142
CGCTCTG92800.030.5808725
CGTAACG4700.030.3804952
Produced by FastQC (version 0.11.5)