FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21564243
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1726630.8006912183284153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT955540.4431131665507572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT577280.2677024183042271No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414430.1921838851472783No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT221810.10286009112399633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA345100.075.864361
GTATCAA794250.067.161931
GCGTAAG23250.061.6771281
ATCAACG977800.054.548383
TCAACGC994800.053.7646264
CAACGCA1002100.053.295165
AACGCAG1030600.051.871386
GCGTAAC9900.048.0822561
TATCAAC1112150.047.8625262
ACGCAGA1154600.046.1715357
CGCAGAG1169550.045.5656558
TCTGTCG71050.045.470488
CGTAACG4050.044.0754052
GTCTCGC67950.042.5575751
GCAGAGT1299500.041.018349
CTGTCGC89800.036.373989
CTCGCTC81850.035.039543
TCTCGCT83700.034.193982
CGTAAGG17500.034.0010262
GCGTACT24350.032.499951
Produced by FastQC (version 0.11.5)