FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15102272
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG4361372.8878899810571546No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA2160461.43055296580541No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1922641.273079971013633No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1882931.24678591406644No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1745791.155978385238989No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1319020.8733917651595733No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1164390.7710031973997026No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1026550.6797321621541448No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG995650.6592716645548432No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG910580.6029423917143063No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG909490.6022206460061108No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA804140.5324629300809839No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG745280.49348866183843065No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA694240.45969242243816033No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA688520.45590491285019896No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG658580.43608008119573005No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC627290.41536134430634014No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA620460.410838845969666No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC525990.34828534408597595No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC437050.28939354290533237No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC429520.28440753815055114No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA427460.2830435049772644No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA417810.27665373792764425No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA405440.2684629173676649No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT389090.2576367317447335No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA385240.25508744644514414No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG374440.24793620456577659No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG371920.24626758146059086No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT371760.24616163713645206No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG367890.24359910879634536No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA362490.24002348785666155No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA349500.2314221330406445No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG348600.23082619621736383No Hit
GGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG341700.22625734723887903No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA337400.22341009352764934No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC337150.2232445555211825No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG331050.21920542816339156No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA314070.2079620867641637No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG300390.19890384705029815No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC294130.19475877536836841No Hit
CATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGG278980.1847271721764778No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG277400.18368097197560737No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA272770.18061520809584147No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG267740.1772845834057286No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA263410.17441746513372292No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC257370.17041806689748404No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC255380.16910038436600797No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA250320.16574989511511912No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA250100.1656042216694283No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA242920.1608499701237006No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC231840.15351332567709017No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT230880.1528776597322575No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC230420.1525730698003585No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC226930.1502621592300814No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA223830.14820948794989258No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG222890.14758706504557725No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA222200.14713018014772877No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG219880.14559398744771648No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA218850.14491197086107308No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC217850.1442498188352057No Hit
CAGCCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG214890.14228984883863832No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG212140.14046893076750305No Hit
CTTCGTTGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT207180.1371846567192009No Hit
TGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAA205310.13594643243082896No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG199180.131887440512262No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG195990.1297751755497451No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG192580.12751723714153737No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA186720.12363702626995463No Hit
TTGTGTAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC184810.12237231590054795No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG184260.12200813228632089No Hit
TTTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG176180.11665794391731256No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT175730.11635997550567226No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG171810.11376433956427218No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA171110.11330083314616503No Hit
GGGGTGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA169570.11228111902632928No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT168730.1117249113246007No Hit
TTGTGGAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA167850.11114221754183742No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCT167310.11078465544786904No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG166820.11046020095519403No Hit
GCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGA165530.10960602484182513No Hit
CGACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG165460.10955967420001442No Hit
GCCCCGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA163050.10796388781767405No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA160070.1059906747805893No Hit
TGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG156890.10388503133833109No Hit
CTTCGCTGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT156690.10375260093315761No Hit
GTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC152870.10122318019434426No Hit
GCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG152270.10082588897882384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTGTT221250.087.804731
GGTGCTA144100.086.457642
GTGCTAG145750.086.03333
TGCTAGT148800.085.930684
GGTGTTA246000.085.058022
TGATAGT235000.084.79154
CGCGATA14200.083.3511053
TGGTGCT146700.082.842911
TTCGACG16800.082.73861
GATAGTA275950.081.71665
GTACGGT21850.081.144242
CGACCTA20800.080.787841
TACGGTG23000.078.947043
GCTAGTA166650.078.63715
CTTCGCT23900.075.288481
TGGGGTA115050.075.262861
CTTCGTT29150.074.79311
TCGACGT20100.074.492892
GACCTAT28350.073.941092
TGCGTAA11450.073.8949663
Produced by FastQC (version 0.11.5)