FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7779995
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT716690.9211959647788978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337860.434267631277398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301820.38794369405121726No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236330.3037662620605797No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG213860.2748844954270536No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC164480.21141401761826323No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC133840.17203095888879105No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA122860.157917839278817No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG111450.1432520200848458No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG96540.12408748334671166No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG93420.12007719799305783No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA89170.11461446954657425No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC88680.11398464909039144No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG86400.11105405594733674No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG85920.11043708896985154No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT85710.11016716591720176No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG84570.10870186934567438No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA84390.10847050672911744No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG82480.10601549229787423No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA81030.104151737886721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80000.092.191061
GTATCAA264200.071.737751
GCAGCGC17800.058.1611488
ATCAACG332650.056.7964673
TGCAGCG18400.056.5879487
CAACGCA338100.056.1798745
TCAACGC338900.056.012494
AACGCAG359250.052.8675736
ACGCAGA378500.050.1293837
CGCAGAG381200.049.789938
GCAGAGT400700.047.337219
TATCAAC412650.046.0167922
CATGCAG23000.045.5495385
GTACTTT678100.041.969381
TGGTATC23100.041.4878962
GCTCTAG19600.041.3038331
TGGTCCT32450.041.0896535
GTGGTAT24200.039.3561061
ACTTTTT757100.037.6524733
TACTTTT789500.036.1378942
Produced by FastQC (version 0.11.5)