FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7779995
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG207940.26727523603806946No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC154960.19917750589814004No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA133190.1711954827734465No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC133020.17097697363558717No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG109430.14065561738792892No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG100360.12899751220919806No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG97710.12559134035433187No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG95940.12331627462485516No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC92670.1191131870907372No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT91340.11740367442395529No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG85440.1098201219923663No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC83990.1079563675812131No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA81670.10497436052336795No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG80380.10331626177137647No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG80040.10287924349565776No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT78500.10089980777622606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.059.5584141
GCAGCGC15250.055.789998
TGCAGCG16650.051.0660827
CAACGCA58900.046.465125
CGCAGAG62800.043.674858
ACGCAGA62900.043.5773667
TGGTCCT34650.043.097795
CATGCAG20100.042.3276825
ATCAACG65050.041.907063
TCAACGC65900.041.183244
GCTCTAG19450.038.889041
GCAGAGT69950.038.8746159
GTATCAA74650.038.2163161
AACGCAG75500.036.4609536
TCCAGTA17850.034.6788062
TAGCGTG8400.034.0016179
GTACACT21200.031.130146
TCTGTCG3250.031.1211538
CCAGTAC21700.030.994723
GCCTTTC54050.030.8015847
Produced by FastQC (version 0.11.5)