FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7840017
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT725670.9255974827605604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342790.437231194779297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308860.39395322739733857No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243810.31098146853508096No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG213340.27211675688968534No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC167160.21321382338839318No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC135350.17263993177565815No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA125340.15987210231814547No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG111680.14244867071079054No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG99000.12627523639298233No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG97170.12394105778086961No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA92580.11808647863901316No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC92240.11765280610998675No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG89840.11459158825803566No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT87960.11219363427400732No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA87120.11112220802582443No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG86750.11065027027364865No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG85970.10965537447176453No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG85090.10853292792604914No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA84630.10794619450442518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83800.092.749141
GTATCAA266000.069.222841
GCAGCGC16200.058.0296948
TGCAGCG17250.054.497457
ATCAACG338100.054.1966483
CAACGCA341800.053.7666745
TCAACGC343950.053.465194
AACGCAG364400.050.4377946
ACGCAGA384700.047.7444237
CGCAGAG386550.047.5159238
CGTCAAT4200.045.3332866
GCAGAGT405650.045.3226439
TATCAAC424600.043.3241162
TGGTCCT33550.043.104415
GTACTTT682450.042.6430971
CATGCAG22250.042.5280085
TGGTATC22900.041.8409082
TCCAGTA17000.039.9086152
GTGGTAT25100.039.5961951
ACTTTTT761850.038.253273
Produced by FastQC (version 0.11.5)