FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18542829
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG281270.1516866708957948No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG190790.10289152750100862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC77650.052.8961839
TTCCGAC83500.048.9614948
CGGGGAT161750.043.6814088
TCGGGGA173800.040.8709837
TGTCGGG45150.038.9154245
TTTCCGA106950.038.358917
TCACCCC301950.037.490479
GTTCACC311450.036.3760347
GTTCCGT33200.035.464431
CTATCGG90800.035.2482724
GTTTCCG96250.034.8285146
TCTATCG89950.034.6833423
TTCACCC326200.034.629688
ATAGCGC173450.034.5037794-95
ATCGGGG119300.034.1091846
TATCGGG100450.033.990445
CCCCCGT42650.033.9515951
CCGTTTC107750.033.7138144
GTCGGGG55550.033.5922746
CGTTTCC100550.033.437135
Produced by FastQC (version 0.11.5)