FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21306431
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC594760.27914576589575235No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG346150.162462685561932No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT339930.15954337917974154No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC281750.13223706964343301No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC260610.12231518267888225No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC256780.12051760334708334No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT227910.10696770378858853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA55050.049.8689544
GCGACAT59400.046.6218765
CTGCGAC73250.037.672833
GCTGCGA82450.033.8148272
ACCCGAC98200.033.7600983
CTAGGCG205100.033.2348637
TAGGCGG212250.032.003398
CCGACTC105350.031.652665
CCCGACT111200.030.1550244
GCTAGGC228050.029.9527346
TGGCTAG234900.029.7028794
CGACTCC111700.029.5980916
AACCCGA115400.029.4279482
GGCTAGG238600.029.0265055
GAACCCG131700.026.841761
GCGCGGT74150.026.3933016
GGCTGCG118800.024.117081
CTGGCTA291700.024.081943
TCGATCG69700.023.98772216-17
GGCTGGC312700.022.9593961
Produced by FastQC (version 0.11.5)