FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8456931
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC119840.1417062525400763No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA111380.13170262356403287No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA100320.11862459324783424No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC96800.11446232681808566No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC93310.11033553424995426No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG92560.10944868770952489No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG86460.10223566918069925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC15800.059.8709838
GCTCTAG14250.055.5916521
TGCAGCG17850.053.6302267
GGTATCA24200.049.963571
CAACGCA59750.047.6990785
CGCAGAG64050.044.7717748
ACGCAGA64300.044.571587
ATCAACG67800.042.4052963
TCAACGC69300.041.399824
GTCTCGC7800.039.708321
TGGTCCT27850.039.0965355
GCAGAGT74350.038.6502959
AACGCAG77200.037.2768946
GTATCAA82150.036.7598041
TCTGTCG8050.036.2140058
CATGCAG27250.035.5904465
CTCGCTC8450.032.4043853
TCTCGCT9450.030.8650462
GCCTTTC44900.030.5905727
CTTTCTA53400.026.9625389
Produced by FastQC (version 0.11.5)