FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23118877
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC52100.039.9180769
CGGGGAT159300.035.0520558
TTCCGAC61200.034.0592428
CCCCCGT51100.033.446441
CCGTTAA27500.033.3256384
CCCCGTT71500.031.937562
TCGGGGA178750.031.7691157
CCGATCG100050.030.96563586-87
GTTCCGT38150.028.8709951
GTCCCGT39350.028.83511
CCCGTTA32200.028.7558983
CGATCGG101800.028.54145686-87
CCCGTTT84950.028.0600573
ATAGCGC103050.027.95504694-95
TGTCGGG47000.027.885795
GATCGGC111300.027.82229888-89
ACCCGAT112150.027.79495284-85
ATCGGGG117050.027.6245826
TCCGTTT106200.027.3190063
CCGTTTC99550.027.2363384
Produced by FastQC (version 0.11.5)