FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22874994
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1423860.622452622282655No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC876150.3830164939059656No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC628920.27493777703285954No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA340510.14885686964551773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA125000.069.25334
GCGACAT129300.067.148915
CTGCGAC133550.065.211193
GCTGCGA144700.060.196762
CGACATC149900.057.9131556
GGCTGCG181000.049.1750261
GACATCT248700.035.2116857
TGGCTAG470150.034.277084
CTAGGCG475500.033.467687
TAGGCGG490900.032.4177678
ACATCTG272900.031.8455878
GCTAGGC500850.031.8307086
GGCTGGC513100.031.3449651
GGCTAGG514000.031.2871655
CTGGCTA530050.030.6097643
GCTGGCT541300.029.8985122
TTGATCG150250.028.81139822-23
CATCTGT303300.028.7318449
TGATCGC152350.028.49318924-25
GATCGCC163150.026.70891824-25
Produced by FastQC (version 0.11.5)