FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22874994
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG501030.2190295656470992No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA409980.17922627651836762No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG268950.11757380133083314No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG59450.049.812565
CTGTCGG70350.041.7567834
TCGGGGA356300.040.725027
CGGGGAT356850.040.4889348
GTCGGGG79800.038.0595366
ATCGGGG260300.033.6524546
TCTGTCG87800.033.1329083
CCCCCGT51350.032.2132031
CCCCGTT66250.031.7844582
GTCCCGT34450.031.0447621
GGTCCGT28850.030.8099381
TATCGGG226550.030.696085
TCTATCG200700.030.4576423
TCCGACC45400.030.2493659
CTATCGG213600.030.0646924
CCGATCG69900.028.70682786-87
CATCGGG42550.028.4867735
GGGGATG512900.028.4710929
CGATCGG69700.028.44849886-87
GCCCCGT48800.028.0522541
Produced by FastQC (version 0.11.5)