FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20193399
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC562610.27861084703966876No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC467230.23137759027095933No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC224830.11133836359099326No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT214010.10598017698753934No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT211430.10470253175307435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA92750.062.2251324
GCGACAT98150.059.146715
CTGCGAC104000.055.539723
GCTGCGA107900.053.7523772
CGACATC109600.052.658066
GGCTGCG147550.040.00081
ACTCGGG152500.032.3143351
GACATCT190450.030.7774987
CTCGGGA169150.029.1774252
TTGATCG101250.028.91990322-23
ACATCTG204150.028.572498
TGATCGC104900.027.70971324-25
TCGGGAG184150.026.7749753
TCGCCAG113300.025.73917226-27
GATCGCC120650.024.13177524-25
CATCTGT244550.023.9493949
ACGGGTG50500.023.797574
CTAGGCG221050.023.2721827
ATTCGGC124800.023.12051638-39
ATCGCCA127400.022.87184726-27
Produced by FastQC (version 0.11.5)