FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23948183
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1211920.506059269715786No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC548200.22891089482655114No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC530930.22169949177355125No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA315080.13156739281639865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA103500.052.3607864
GCGACAT108650.049.9267775
CTGCGAC121750.044.5120473
TCGACCG37950.044.1798063
GCTGCGA132500.041.008232
CCGCTCG43100.038.793827
GCTCGCG43650.038.6315549
CGACATC140000.038.57716
CGACCGC47900.035.8949974
CTCGACC48050.034.992162
TGGCTAG405850.034.1492084
CTAGGCG403250.034.0895547
TAGGCGG417950.032.8223768
GCTAGGC425850.032.3138736
GGCTAGG428400.032.1991425
GGCTGCG174350.031.829951
CCTCGAC55350.030.302061
CTGGCTA465300.030.2147883
CGCTCGC56250.030.0624688
GGCTGGC491150.028.0736141
Produced by FastQC (version 0.11.5)