FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23948183
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG491920.20541015575169108No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG257640.10758227461348528No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA246980.10313099745396133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG66650.045.7447745
CGGGGAT324950.042.7455148
TCGGGGA339350.041.043777
TCTGTCG76750.037.930123
CTGTCGG77950.037.7737774
TCCGACC55600.037.4094669
GTCGGGG92500.034.550666
ATCGGGG242550.033.851416
TCTATCG176600.033.4527473
CCCCGTT72550.033.3772472
TTCCGAC62350.033.359458
CTATCGG190800.032.1338464
TATCGGG205050.031.6140045
CCGTTAA27200.031.253524
CCGTTTA43000.030.041064
CCCGTTT84100.029.758893
GGGGATG473200.029.7350759
CCCGTTA31900.029.3276773
GTCCCGT42650.029.167251
CCGATCG112800.028.75589886-87
Produced by FastQC (version 0.11.5)