FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13612532
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN417290.30654840701200925No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG385030.28284965647830984No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA271970.19979383703193498No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG214440.15753131011923424No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191000.14031188319704224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG42250.057.0184485
CTGTCGG50850.046.6278464
GTCGGGG55500.044.176136
TCTGTCG56900.040.9184883
TCGGGGA257900.038.8008467
CGGGGAT261600.038.2564438
TAGTAGC178650.035.852637
GTAGCAA182150.035.352089
AGTAGCA194450.033.163168
GCCCCGT31950.032.6057661
ATCGGGG192600.030.6158586
TCAGTCG137750.030.37470884-85
AAACGAG144600.030.23520322-23
CGAGAAC140500.030.0366724-25
CCCCCGT27100.030.0156671
ACGAGAA145700.029.92581224-25
AGTCGGT139100.029.73787986-87
TATCGGG162150.029.6842925
AAGGCCG147650.029.35321436-37
TCTATCG145700.029.2854943
Produced by FastQC (version 0.11.5)