FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10609722
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG297220.2801392911143195No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA221860.20911009732394498No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG166900.15730855153414952No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151470.14276528640429975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG32350.052.497355
CTGTCGG36950.046.3476334
TCTGTCG38300.043.990553
GTCGGGG41400.042.718866
TCGGGGA206250.038.96617
CGGGGAT209250.038.2714848
GCCCCGT23500.037.359831
GTAGCAA136000.035.8397839
TAGTAGC136950.035.660417
AGTAGCA150150.032.557018
ATCGGGG154300.032.326676
TATCGGG131300.030.9269925
ATAGCGC45350.030.7983294-95
CCCCGTT31500.030.7341122
TTATCGG11450.030.2449024
CGAGAAC108350.030.03284624-25
TCTATCG118700.029.9875033
CCCCCGT22350.029.7270321
TTCCGAC26150.029.5547358
TCAGTCG107400.029.4869684-85
Produced by FastQC (version 0.11.5)