FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22866979
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG7303893.194077363695484No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA3189781.3949284686884087No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA2808821.228330161146341No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2782201.2166889207358786No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2767751.210369765066037No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1626500.7112876606918649No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1612640.7052265189905496No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1446580.6326065196456427No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1368410.5984218553749492No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG1319450.577011069105368No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1269110.5549967925365218No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1113600.48699043279831583No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG1112550.48653125539670106No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA1078740.4717457430647048No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1031560.45111337181881356No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1011990.4425551796763359No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA956500.41828874728052184No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC926230.40505131875968403No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG891730.38996406127805516No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC785430.34347781576219577No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA778620.34049972232886555No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT732060.32013848440583254No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG719170.3145015351612471No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA696130.30442587103438545No Hit
CATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGG679490.29714900249831866No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG663560.2901826253481057No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA662920.2899027457890262No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA656640.28715642761555865No Hit
GGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG642820.28111277838668586No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA617080.2698563723699576No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG568230.24849369039959324No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC533810.2334414178628493No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA526040.23004350509089982No Hit
TGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAA514490.22499255367313714No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA512470.2241091838147925No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA504540.22064130115307315No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC489790.214190951939913No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG477320.2087376736559735No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA446550.19528158922960484No Hit
GTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC445190.19468684516656093No Hit
TTGTGGAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA440080.1924521818120356No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC437570.19135452916627071No Hit
TGTGAGGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAA435590.19048865178036853No Hit
TTTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG434590.19005133996930684No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT427350.1868852024572201No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT407450.17818269741709214No Hit
CTTCGTTGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT397670.17390578790490863No Hit
TGTGGAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAA395100.17278189655048007No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC380930.16658518818773568No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG374530.16378639259694078No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG366780.16039722606121254No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA362950.15872232182484622No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG356470.15588854128916638No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC353490.15458535209220248No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT351310.15363201234408796No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC347430.15193524251716853No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA344440.15062768020209402No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG342930.14996733936739085No Hit
TCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAG339350.14840176308378994No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC338480.14802130180816628No Hit
GTTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG336000.14693676851673323No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA334150.14612774166626907No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG333230.1457254148000923No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA331710.1450607008472785No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT329800.14422543528815066No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG329680.14417295787082324No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCT320020.13994852577596717No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA317850.1389995591459633No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGG315790.13809869681517614No Hit
CCGTGTGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG315490.13796750327185764No Hit
GCGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA315080.13778820542932232No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG309530.1353611248779299No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA307260.1343684270668198No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC303900.13289905938165247No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA294170.12864401546002208No Hit
GCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATG289070.12641372522360736No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA285470.12483940270378523No Hit
CCGTGAGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG285120.12468634356991362No Hit
ATGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA283190.12384233177456454No Hit
CTTCGCTGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT280740.12277091783746337No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA279610.12227675549096363No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC278240.1216776383098091No Hit
GCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGA277080.1211703566089775No Hit
TGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACC275800.12061059749081852No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA266570.11657420947471898No Hit
GCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG263260.11512670738010473No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG262810.11492991706512697No Hit
TGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG252810.11055679895450991No Hit
TGTGATGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAA248400.10862825386772777No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC248190.10853641838740483No Hit
TTGTGTAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA243910.10666472383606072No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT241480.10560205613518078No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCT239080.10455250778863268No Hit
TTGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA236760.10353794438696952No Hit
CAGCCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG236240.10331054224521745No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG235810.10312249816646091No Hit
TGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG234200.10241842615065155No Hit
GGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTA231550.10125954985133803No Hit
GGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTAC231040.10103652082769655No Hit
CCGTGCGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG230340.10073040255995337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTGTT315600.090.712971
GGTGCTA196500.088.510992
GTGCTAG199700.087.997563
CGCGATA24150.087.981143
TGCTAGT205800.087.583484
TGATAGT377850.087.078594
TGGTGCT194900.086.724831
TTCGACG20950.085.536381
GGTGTTA362100.085.208012
GATAGTA433150.085.069665
CTTCGTT53650.083.459291
CGACCTA22400.083.182431
CTTCGCT37050.082.7494351
GTACGGT25700.082.119672
TGCGTAA18550.081.7421343
CCGTGCG36700.080.559691
GTCAACT31600.079.5693746
CTGCGTA18850.079.432192
GCTAGTA231750.079.2121355
CGTGAGT51900.078.580952
Produced by FastQC (version 0.11.5)