FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12151988
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC270730.22278659261348843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA46100.053.9174084
GCGACAT47600.052.4168785
CTGCGAC62200.040.1132353
GCTGCGA67100.037.2543752
CGACATC73200.034.2786756
TAGCCCC45100.029.4764081
TAGCCCA57300.026.2553221
GGCTGCG100300.025.847851
GGTCGAC142600.025.7142516-17
TAGGCCC41650.025.4435561
TAGCCCG14800.024.9331821
TTGATCG50300.024.61302422-23
CAAGCTT167050.023.9879742
GTGTGCT119200.023.9046088
TGATCGC52350.023.87524224-25
ACATCTG105950.023.727468
TCGACCC154000.023.65730918-19
AAGCTTG172000.023.5723573
GACATCT108150.023.4195677
TTGGGCT177300.023.3472637
Produced by FastQC (version 0.11.5)