FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12151988
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA108900.035.8964429
AGTACGA35650.035.0518659
TTCCGAC31550.034.0679178
CAGTACG37200.033.709238
TAGTAGC116150.033.603297
TCGCGCG51150.032.991967
TCCGACC33050.032.5303049
AGTAGCA122300.031.9164078
ATTGCGC30700.031.1804549
CGAGAAC84200.029.70141624-25
ACGAGAA85900.029.44413824-25
AAACGAG86150.029.42116422-23
CGGTGAA52550.029.321635
CAAACGA86800.028.8048320-21
CGAAGTG85850.028.42915540-41
GTACGGT12000.028.2586422
TCAGTCG70800.028.25449884-85
CGGGGAT73900.028.0046548
CATTGCG34450.027.7790188
AGTCGGT68400.027.71226986-87
Produced by FastQC (version 0.11.5)