FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9254335
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGG228580.24699775834784457No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC209590.22647764534134543No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG169120.18274678839700528TruSeq Adapter, Index 11 (100% over 49bp)
CAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACG121100.1308575926849417No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC108560.11730718630782223No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC101120.10926771075393316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)