FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8514421
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT777810.9135207197295035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381570.4481455638615944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374880.440288306157283No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182200.21398988844925568No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC112350.13195260135715628No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA110470.12974458274966671No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA105580.1240013854142284No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG94660.11117608584306554No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA93660.11000160786035831No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC91940.1079815057301019No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC90220.10596140359984549No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA86470.10155711116469342No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG85360.1002534406038884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99500.091.803521
GTATCAA298100.066.933821
GCAGCGC16150.061.8937848
GCTCTAG12950.060.1971051
TGCAGCG17550.056.6173637
ATCAACG381250.052.63133
CAACGCA384750.052.1525235
TCAACGC387150.051.8753364
AACGCAG407600.049.2525636
ACGCAGA435650.046.080557
CGCAGAG436750.045.9644938
TCTGTCG8950.044.5412148
TATCAAC454250.044.3961752
GCAGAGT460500.043.5422179
CATGCAG26150.038.912655
TGGTATC24900.036.80362
CGTCAAT5600.036.125086
GTGGTAT28500.035.0782931
TGGTCCT31900.034.8832785
GTACTTT714950.033.0853351
Produced by FastQC (version 0.11.5)