FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11103960
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2131591.9196664973576996No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1516171.3654317919012675No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1271241.1448528272796372No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG917280.8260836674483697No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC745920.671760344958015No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG673290.6063512476629959No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT627730.5653208404929413No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC590640.5319183426453264No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT517670.4662030482818742No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA459150.4135011293268347No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC408390.36778770816897755No Hit
TCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGA393370.3542610023811325No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG372050.335060645031142No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC363160.32705449227122574No Hit
CCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAT352270.3172471802852316No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC345900.31151048815017346No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG337460.3039095962161247No Hit
CACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTT288570.25988025893465033No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC286450.2579710301550078No Hit
ATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGA273490.24629951837002295No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG262490.2363931426265945No Hit
TTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGA262260.2361860093155955No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC251960.2269100393012943No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG248960.22420830046217746No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG245050.22068703417519514No Hit
GACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGC242510.21839956195807622No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG242310.21821944603546842No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA225790.20334187082806493No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC225700.20326081866289145No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT202120.18202515138743294No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA200490.18055720661817945No Hit
GCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGT198770.17900820968375247No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG197470.17783745618680183No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA195590.1761443665142886No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA194460.17512671155155457No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC189540.17069585985540295No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC189410.17057878450570788No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG188820.17004744253401488No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA183550.1653013879732996No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA182770.16459893587512925No Hit
CCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATT177000.1594025915078945No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA175080.1576734786508597No Hit
AGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTT173910.15661980050360413No Hit
TACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCC171250.1542242587329205No Hit
CGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCAT168340.15160357205897718No Hit
TACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCT166980.1503787837852442No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAA163990.1476860507422577No Hit
CCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACG162370.1462271117691346No Hit
CCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTC162100.1459839552736141No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT160510.14455203368888217No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG159860.14396665694040683No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA157380.14173321950007026No Hit
GCCCCGGGCGTGGGGGGGGCGGCGGCGCCTCGTCCAGCCGCGGCGCGCGC155790.1403012979153383No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC146080.1315566698727301No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG145410.130953281531994No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT145220.1307821714055166No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG140110.12618020958288756No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA139860.1259550646796278No Hit
TCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTG139170.12533366474663094No Hit
CGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTT138300.12455016048328704No Hit
CAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACG137820.12411788226902834No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA134270.12092082464274007No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC133600.12031743630200396No Hit
GTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAA132030.11890352630953283No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT130980.11795791771584191No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC130660.11766973223966946No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC126300.11374320512681961No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA125530.11304975882477962No Hit
CGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCG124840.11242835889178275No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT120020.10808756515693499No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT119480.10760125216589397No Hit
CACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGA118010.10627740013472672No Hit
AATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCT115150.10370174244143532No Hit
GGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGG114740.10333250480008933No Hit
CTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAAT114530.10314338308135115No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT114480.10309835410069922No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT113070.1018285368463143No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGT112910.10168444410822805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA13650.062.6274454
GCGACAT14000.061.0573585
CTGCGAC16400.052.4154783
TGCGGCG28800.049.799356
GCTGCGA20700.041.9874272
CGCAGGT91700.041.7449762
CTCCGCA15400.040.4136431
CTGCGGC35650.039.829535
CTCAGGA552500.039.675531
GGACCGA563900.038.9833035
CCCGCCA31800.038.6945721
ACCGACT573900.038.396447
CCGACTG583300.037.859098
CGACTGA583550.037.802189
GGTTCAC104550.036.7934236
TCAGGAC601700.036.3962
TCCGCAG46550.036.3219262
CGTCAGG71300.036.0356674
TAGCCGC4500.035.8958131
AGGTTCA109000.035.2900355
Produced by FastQC (version 0.11.5)