FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14237648
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA2051731.441059646930448No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1694301.190013968599308No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1403800.985977459198317No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG1331140.9349437491360933No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA982930.690373859502637No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG965130.6778717945548309No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG945580.6641405940082238No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC879810.61794616638928No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG718630.5047392659236976No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG664050.4664042825050879No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG503960.3539629579267587No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG500860.35178563200888235No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC491330.3450921107194109No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG488650.3432097773452469No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA484780.3404916317638981No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT464360.32614937523388693No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG447240.3141249172616151No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG433720.3046289668068771No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG429320.3015385687298913No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN428350.30085727642655585No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC418630.2940303061292146No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC395840.27802344881682706No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA366780.25761277424473483No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG361660.25401667466424227No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC360810.25341966594482457No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG346290.24322135229077163No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC343450.24122664080471717No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA335060.23533381356246483No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC324460.2278887636497264No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA309680.21750783556385156No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA309370.21729010297206391No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG306140.21502146983827666No Hit
CAGCCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG303350.213061876512188No Hit
GCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGA303340.21305485288019482No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT302500.21246486779277024No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA301310.21162905558558548No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA296240.20806807416505874No Hit
GGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTG292160.20520243231185376No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG280530.19703394830382098No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC279140.1960576634567732No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA274040.192475611140267No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC255160.17921499393720086No Hit
GGTACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC251230.17645470656389314No Hit
GGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGC249820.17546437445285906No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA243170.1707936591774147No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG242350.17021772135397645No Hit
GATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGC230660.16200709555398476No Hit
GGCATGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC230350.16178936296219715No Hit
CGACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG228830.16072177089923842No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT227280.15963310794030025No Hit
GGCGCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC222400.15620557552764333No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC222070.1559737956718694No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA211810.14876754924689808No Hit
CAACCCATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG210350.14774209897589827No Hit
GCCCTGTCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG206860.1452908514102891No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA198900.13970004034374217No Hit
GGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTAC193630.13599858628335243No Hit
GCCCCGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA190040.13347710239781177No Hit
GGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTT187470.13167202897557237No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA186850.1312365637919971No Hit
CTTCGTTGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT182360.128082953027073No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA180250.12660096667651846No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG180180.12655180125256643No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA178420.12531564202177214No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC176340.12385472656719705No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA175930.12356675765547794No Hit
GTCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA175410.12320152879183417No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA172180.1209328956580469No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA168700.1184886717244309No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA165490.11623408585462991No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT163900.11511732836771917No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG163820.11506113931177397No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG163760.11501899751981508No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT161330.11331225494547975No Hit
GGGGTGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA160910.11301726240176749No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT156130.10965996630904207No Hit
GGGTGTAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC155700.10935795013333663No Hit
CTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCACG153850.10805857821460398No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGG152910.10739835680724794No Hit
CATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGG151980.10674515903188506No Hit
TAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG150440.10566351970494002No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC149260.10483473112974839No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT149240.10482068386576208No Hit
GGCCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG145910.10248181441204333No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA144740.10166004946884485No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA143670.10090852084557787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA27250.084.315881
GATAGTA145650.083.118645
GTACGGT32500.082.979752
TTCGACG17300.082.4904861
TACGGTG33850.080.987113
CGCGATA8900.080.075563
GGTGTTA127900.079.664772
TGATAGT137000.079.555754
GGTACGG34050.079.492081
GTGCTAG85100.079.390543
TGCTAGT86300.079.222544
GGTGCTA85500.078.910562
GCTAGTA89950.076.854855
TGGTGTT129400.075.765661
TCGACGT19450.075.6744462
GACCTAT41950.073.907472
CGTGAGT22900.073.189092
GTGATAG148700.071.2512743
GGTGATA144850.071.129272
ATACCGG113750.070.8956769
Produced by FastQC (version 0.11.5)