FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12013485
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA537890.44773852050425No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG482740.40183177487631605No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA403710.3360473667715904No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG312900.26045731109665515No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG280450.23344599839263963No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA277220.23075735309113052No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC262670.21864596326544714No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG260440.21678971589010185No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC245140.2040540276197956No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG232690.1936906734390562No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG199780.1662964576890053No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG169130.14078346125208463No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA149780.12467656138081498No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG140310.11679375302004373No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG133950.11149970220964191No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG133020.11072557213830958No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC130720.10881105690813282No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG130150.10833659009021944No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG122870.10227673318774695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA12200.063.0124631
GTACGGT20800.062.0550042
AATACCG45100.057.826118
ATACCGG45900.056.6116379
GCTAGTA41200.055.1505785
TACGGTG24450.053.3783263
GATAGTA64450.052.9197435
GGTACGG24400.052.8993531
AGTACGA139900.052.4675879
GTAGCAA491000.052.3040129
CAGTACG140600.052.2400938
GGTGTTA62100.051.5802462
ATAGTAG80850.051.3761946
GTACACC109400.050.1046837
AGTAGCA517100.049.5173158
GCGGTAC88950.047.730914
GGTACAC103150.047.072646
TACACCT117400.046.8115658
TGCTAGT48850.046.4179534
TCAGTAC163200.045.2092447
Produced by FastQC (version 0.11.5)