FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14702119
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA644740.4385354247234702No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG581900.39579328666840474No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA513930.3495618556753622No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN443600.30172521389603774No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG398810.27126021765978087No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG339550.2309531027466177No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA338530.23025932520339415No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC332020.22583139205987926No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG320930.21828826171247834No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC297140.20210692077788245No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG291480.19825713558705382No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG244020.1659760746053001No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG207200.1409320656430546No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA185720.1263219267916414No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG166420.113194567395353No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC165140.11232394459601366No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG162900.11080035469716984No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG159250.10831771937092878No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG158680.10793002015559798No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG147430.10027806195827962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT25250.060.359462
AATACCG52750.059.3666238
CGACCTA15600.057.9408341
ATACCGG54000.057.8199589
GCTAGTA50850.055.9694825
GATAGTA82200.054.6229865
AGTACGA169600.053.4916849
CAGTACG171700.052.9172178
GGTACGG29400.052.1031881
GTACACC138150.051.7271237
GTAGCAA604600.051.602749
ATAGTAG105150.051.1051066
GGTGTTA76750.049.9225582
TGCTAGT56100.049.2925764
TACGGTG30850.049.285163
AGTAGCA635600.049.0380448
GGTACAC128750.048.0110326
GCGGTAC111450.047.6631934
TACACCT151500.047.4262288
GTGATAG81000.046.3994523
Produced by FastQC (version 0.11.5)