FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8096474
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC110900.13697320586714662No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA106370.1313781777104453No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA95470.11791552717886823No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC92460.11419785946326759No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG89560.11061605335853607No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC87870.10852872497336494No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG82830.10230379298445225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC13650.063.627258
TGCAGCG14500.060.306147
GCTCTAG12150.051.9538081
CAACGCA60800.048.762645
GGTATCA23600.046.9341241
CGCAGAG64900.046.013158
ACGCAGA65450.045.625367
ATCAACG69650.042.224773
TCAACGC70800.041.6229974
GCAGAGT73900.040.329149
AACGCAG76400.039.2430576
GTATCAA82300.037.7709961
TCTGTCG9450.036.5106168
GTCTCGC8850.036.3360941
TGGTCCT27500.034.6376765
CATGCAG26250.033.3387645
CTCGCTC9600.032.8674553
TCTCGCT10000.032.148092
CCAGTAC23400.027.7314193
TCCAGTA20650.026.5234012
Produced by FastQC (version 0.11.5)