FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004913372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10025926
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG5726235.711422565855762No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1016001.0133727298605635No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT598900.597351307001468No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG583620.5821108194893918No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC534540.5331577352555764No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGG499390.498098629493176No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC465410.46420649823268195No Hit
CCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCC401290.4002523058717968No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA354290.35337384297470376No Hit
AGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCG348790.34788806540163975No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCC289000.28825267611191224No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT262750.2620705558768337No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATT261790.261113038336808No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC224600.22401920780185294No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCA220230.21966050816652746No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT214710.21415478231137952No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT211430.21088326404962496No Hit
AGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGA204180.2036520117942223No Hit
ACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAG194950.19444587961251658No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAA194820.19431621577897146No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG194560.19405688811188113No Hit
CACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT193660.19315921541810702No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA193020.19252087039142318No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC191430.1909349819657556No Hit
GACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGC190850.19035648178532336No Hit
AGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGAC189360.18887033477007512No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCG189100.1886110071029848No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGC185150.1846712213914206No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA182290.18181861705342728No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC177420.1769612103660051No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCA175440.17498633043970202No Hit
ACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTT172110.17166494147273778No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA170620.1701787944574895No Hit
ACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGT167410.16697709518302847No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA167340.16690727619573495No Hit
ACCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGG166960.16652825883614142No Hit
CCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTC166140.16571037927070276No Hit
ACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAG163010.16258847312457722No Hit
CCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCC157140.15673365233296158No Hit
CCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCC155180.15477872068874238No Hit
CAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCG153220.15282378904452318No Hit
CGCAGACCCGACGCACCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATG152190.1517964525172039No Hit
CAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCC146480.1461012179822592No Hit
AGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGA145410.14503398489077218No Hit
CCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCC140990.14062541455023705No Hit
CCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGC137960.13760324981453084No Hit
CCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCC131540.1311998512656088No Hit
ACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAA130810.13047173896954756No Hit
ATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCG129630.12929479032659927No Hit
CAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAG129500.12916512649305412No Hit
CCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGGG128150.12781861745239292No Hit
AACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGA125990.12566420298733505No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA124010.12368932306103196No Hit
CCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGG119880.11957000281071295No Hit
CCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACG115860.11556039811185521No Hit
CCGCCCCCGGGGCCGCGGTTCCGCGCGGCGCCTCGCCTCGGCCGGCGCCT115120.11482231167475204No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA114830.11453306158453593No Hit
CAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGA114710.11441337189203273No Hit
CCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTAC113140.11284743174844897No Hit
CCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGG106950.10667343844349139No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGA106680.10640413663535918No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGC106310.10603509341680759No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)