FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10025926
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC691590.6898016203191606No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA665640.66391872431534No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG414150.4130790512517248No Hit
CTCTCCTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC333830.3329667504029054No Hit
CTCTCATCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC291700.2909456941932346No Hit
CGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTTTCCTCCGCTGACTAAT288060.2873151068539704No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA281490.2807620961894193No Hit
GGTTAGTTTCTTTTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGTCG264040.2633572200712433No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT251920.25126856112841844No Hit
CGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAAT229860.22926560598991058No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGT226550.2259641653050302No Hit
CGTGCTGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA202950.20242519244606433No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC183160.18268636732407562No Hit
CTCTTTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC172490.1720439588323313No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG158700.15828961833550337No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG158380.15797044582216146No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT157420.15701292828213576No Hit
CCTGGTTAGTTTCTTTTCCTCCGCTGACTAATATGCTTAAATTCAGCGGG156320.15591577276752291No Hit
GGTTAGTTTCTTCTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGTCG147720.14733801147145908No Hit
CGTGTCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA144610.14423605360741742No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT139510.13914924167603074No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA138750.13839120695684368No Hit
GCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACG137730.13737384457056637No Hit
GCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGA134190.13384299864172147No Hit
TGGTTAGTTTCTTTTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGTC133670.13332434330754087No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT131680.13133948924019587No Hit
CTCTCGTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC129290.12895566953117346No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA125160.12483634928085446No Hit
TGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA120110.11979940805467745No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC119650.11934059756674846No Hit
CTCTGTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC116770.11646804494667125No Hit
GCCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCT112930.11263797478656835No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA112730.11243849196572965No Hit
GCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG108420.1081396371766558No Hit
GGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCG108190.1079102319326913No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCAT107740.10746139558580425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)