FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10312705
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC308920.29955283313155956No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC245560.23811405446000833No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG177100.17172991955069014No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG173430.1681712024148853No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT126290.12246059593482021No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC121180.1175055429201165No Hit
CCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGG114640.11116385080345069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA54450.059.4896854
GCGACAT55350.058.6064765
GCTGCGA68850.047.6031952
CTGCGAC69650.046.9161643
CGACATC80600.040.6587946
GGCTGCG93550.035.605041
CGCACTA58000.030.7891867
ACATCTG107400.030.3359898
GTCCGCA67100.026.897774
CATCTGT127250.026.0143729
TAGCCGC13550.025.4661711
TCCGCAC71250.025.3303555
AAGCTTG123350.025.1822053
TAGCCCC36500.024.9477391
GACATCT132000.024.8983467
CAAGCTT124300.024.8771632
TCACGAA23000.024.7804154
GCACTAA74400.024.3853388
CACGAAT24000.024.3408895
GGTCGAC107100.024.01304816-17
Produced by FastQC (version 0.11.5)