FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10312705
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA139350.1351245866142782No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG134910.13081921765433996No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG119350.11573103274068249No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111790.10840026937646331No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA104760.10158343518989439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA148950.042.8649379
TAGTAGC156950.040.692087
CAGTACG47400.039.2844168
AGTAGCA164700.038.840778
AGTACGA48300.038.3680239
TTCCGAC35300.037.5260778
TCCGACC35950.036.7268079
TCGCGCG44150.035.8707547
TGTCGGG26600.034.8930785
CGGGGAT109200.034.794778
CGGTGAA62200.033.9345635
TCGGGGA113150.033.6996887
GCCCCGT32900.033.200381
CGAGAAC127000.032.31945824-25
CGAAGTG127100.031.99442340-41
ACGAGAA129250.031.86665724-25
AAACGAG130550.031.7483622-23
CAAACGA129450.031.73279820-21
GTAGTAG127900.031.723126
CCCGCGA53650.031.31257864-65
Produced by FastQC (version 0.11.5)