FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18697240
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG364910.19516784295436118No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA217230.11618292325498308No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG207310.11087732734884935No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG202630.10837428411893948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC90700.050.9936839
TTCCGAC97200.047.5339938
TGTCGGG51000.043.205525
CGGGGAT241300.043.0180328
TCGGGGA248100.041.9722867
TTTCCGA119300.039.046257
TCACCCC360900.038.1308259
GTTTCCG107800.037.397256
CTGTCGG59100.037.2820554
GTTCCGT31000.036.144081
GTCGGGG65050.036.133396
CGTTTCC108150.035.611135
CCGTTTC113550.034.7940944
TTCACCC395850.034.727678
GTTCACC397350.034.584077
ATCGGGG177000.034.4462936
TCTGTCG63250.034.3849563
TCTATCG130750.033.5941433
ATAGCGC222700.033.53402394-95
CTATCGG139100.032.806934
Produced by FastQC (version 0.11.5)