FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20874044
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC775630.37157629829658306No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC359820.17237675651157963No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG297350.1424496374540554TruSeq Adapter, Index 18 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA134550.059.1691254
GCGACAT134950.059.070225
TCGACCG34800.052.9610063
GCTGCGA155100.051.7275122
CGACATC155950.051.1713186
CTGCGAC156500.051.1130183
CCGCTCG36900.050.461767
GCTCGCG40500.045.858999
CGCTCGC42500.043.7009168
GGCTGCG184350.043.640561
CGACCGC45950.041.2469864
CTCGACC46450.039.8825262
CCTCGAC48850.038.3272931
GACCGCT52250.035.8227925
GACATCT245400.032.8287287
CGCGTCG29200.031.47422412-13
CTCGCGT30200.030.59249310-11
ACATCTG265800.030.291278
CGTCGCA31200.029.38053114-15
TCGCGTC31850.029.0822110-11
Produced by FastQC (version 0.11.5)