FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20874044
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC285860.13694519375354386Illumina Single End PCR Primer 1 (100% over 50bp)
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG265460.1271722910998942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC51650.043.5774429
CGGGGAT169200.040.4675758
TTCCGAC58400.038.7022638
TCGGGGA181950.038.1014027
TGTCGGG43000.036.1135755
GTCCCGT34400.033.125051
TCTATCG93400.032.6920173
CTATCGG95400.032.454964
CCCCGTT63500.032.0010642
ATCGGGG128000.031.4948046
GCTCCGT65050.030.4363441
CCGTTTA34950.030.4356584
CCCCCGT43200.029.1249961
TATCGGG112450.029.0127225
GTCGGGG57350.028.8957026
CCGATCG87750.028.85620786-87
GACGTAT46800.028.7704644-45
TCCCCGT61850.028.6333981
CTGTCGG55600.028.3560094
CCCCGAA56550.028.2938272
Produced by FastQC (version 0.11.5)