FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18523271
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA268700.053.3008814-15
GGTGTGC239650.048.1019866
GTGTGCT328150.047.3130267
TGTGCTC349000.043.9439248
CGGTGTG86550.043.7701725
GTGCTCT358800.042.8049169
TTCCGAC71000.036.3421068
TCCGACC72550.035.4441879
CGGGGAT116700.033.2411358
GGGTGTG188550.032.9645655
TCACCCC237000.031.5099079
TGGTCGT124200.031.15912414-15
TCGGGGA125900.030.9177
CCGGTGT67750.030.6237584
GCGGTGT28900.030.4844974
CCCGTTA18150.030.0953223
AAACGAG87700.029.6481322-23
GCCCCGT35700.029.1203481
CCCCGTT47850.028.9999872
CGGTTCA110650.028.9420915
Produced by FastQC (version 0.11.5)