FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11085066
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC267850.2416313984959584No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC264580.23868148371872572No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG169940.153305356955024No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC138720.1251413388066431No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA48350.054.02814
GCGACAT50700.051.4285355
CTGCGAC64900.040.32373
CGACATC67300.038.884486
GCTGCGA69100.037.53182
GGCTGCG99250.027.1868691
CGCACTA65250.026.7131637
GCGCGGT40200.025.8736576
CAAGCTT139000.025.4922892
TCGACCG18700.025.398723
TTGGGCT141400.025.3928897
AAGCTTG144800.024.403823
GTCCGCA72500.024.304654
GACATCT109850.024.2983077
GGTCGAC118250.024.23895616-17
TAGCCCC37800.024.2171421
ACATCTG111350.023.8856778
TAGGCCC35750.023.8630141
TCCGCAC77150.022.7774735
CTTGGGC160950.022.6035966
Produced by FastQC (version 0.11.5)