FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11085066
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA136410.12305745405575394No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134800.12160504953240693No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG134170.12103671732761898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC40650.042.559068
TCCGACC42800.040.4319469
CGGGGAT107900.035.1253858
TTTCCGA51600.034.069917
TCGGGGA112300.033.911237
TCGCGCG42600.033.6337937
CCGTTTC53750.032.953494
TGTCGGG27700.032.4854435
GTTTCCG51550.030.4404356
CTCCGTT48050.030.3823262
TCACCCC144500.030.11879
ATCGGGG77600.029.2698576
CCGATCG54150.029.23363786-87
CGATCGG52800.028.8053686-87
CGTTTCC54850.028.7742085
CGCGCGT49600.028.7019738
CCGTTTA18800.028.5653154
ATAGCGC50400.028.38061394-95
TCCGTTT57600.028.374513
CCGTTAC9300.028.3659344
Produced by FastQC (version 0.11.5)