FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12465121
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC580130.4654026222449024No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG150110.12042402155582765No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC141200.11327607650178446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA85250.067.461054
GCGACAT91400.063.076935
CTGCGAC101000.057.268653
GCTGCGA107100.053.7412532
CGACATC114050.050.7557836
GGCTGCG143800.040.5349771
ACATCTG151250.038.334868
CATCTGT166050.035.0603759
GACATCT168850.034.395027
TAGCCCA55400.031.5987631
TTGATCG94950.030.58130322-23
TGATCGC97050.029.96838224-25
TAGCCCC46350.028.2753621
GGTCGAC131050.027.25924316-17
GATCGCC115050.025.3207924-25
TTCGGCT117050.024.82763340-41
ATTCGGC118500.024.38412338-39
TGATTCG117750.024.37891636-37
ATTGATC120750.024.34065422-23
TCGACCC148150.023.92151518-19
Produced by FastQC (version 0.11.5)