FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20091538
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC439070.21853478812821595No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC302800.15071021441962282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT58650.054.426935
TGCGACA60450.052.5646444
CTGCGAC70450.045.1707953
GCTGCGA80250.040.1280982
CTAGGCG165700.029.7282127
CGACATC108050.029.7190196
TAGGCGG170450.028.9555028
ACCCGAC55500.028.925953
GGCTGCG116300.028.435651
GCTAGGC189700.026.1924976
CCGACTC62350.025.3699935
CCCGACT63500.025.3565354
GGCTAGG203800.024.9863725
TGGCTAG210850.024.3960084
CGACTCC66600.023.679716
ACTCGGG136700.023.114621
AACCCGA71750.022.9043562
TCGATCG38250.020.92436816-17
CTCGGGA154050.020.59582
TGATCGC78300.020.4424624-25
Produced by FastQC (version 0.11.5)