FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20091538
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC65000.042.4473958
TCCGACC66150.041.565889
CGGGGAT142350.035.3286258
TCGGGGA152350.033.197157
TTTCCGA87650.032.0747647
ATAGCGC108950.031.5896494-95
CCGTTTC94700.030.7417814
GCCCCGT45200.030.673281
CGATCGG112600.030.5601386-87
CCGATCG117600.030.47326786-87
GTCCCGT34050.030.3987141
CGTTTCC89300.030.101335
CCCCGTT57650.029.4849662
TCCGTTT102850.029.4133153
GTTTCCG80800.029.3868036
ACCCGAT121650.029.30206584-85
GTTCCGT32650.029.2299921
AGTCGGT112200.029.20763486-87
CCCGTTA24950.029.122573
GTAGCAA173350.028.9286619
Produced by FastQC (version 0.11.5)