FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18033202
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC885220.49088342713623456No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC676980.37540753993661247No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT314730.17452807327284417No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC240870.13357028884831434No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT212790.11799901093549553No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC207290.11494908114487931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA111550.078.333944
GCGACAT112950.077.615285
CTGCGAC122800.071.5443043
GCTGCGA133650.065.949392
CGACATC146750.059.7716
GGCTGCG159850.055.6129651
ACCCGAC66350.049.3151133
CCCGACT71050.046.0528874
CCGACTC71950.045.6088335
CGACTCC74550.043.7633746
GACATCT211700.041.5678447
ACATCTG213000.041.293918
AACCCGA87450.037.7421572
CATCTGT242000.036.325849
TTCGGCT125450.035.03562540-41
ATTCGGC125800.034.95702738-39
TGATCGC128600.034.23307424-25
TGATTCG129400.033.87439736-37
TCGGCTG130250.033.65333640-41
GAACCCG105350.033.1116831
Produced by FastQC (version 0.11.5)